| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173365 |
| Chromosome: | chromosome 6 |
| Location: | 6867275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295450 | HPR1 | (1 of 1) 1.1.1.29 - Glycerate dehydrogenase / Hydroxypyruvate reductase; Hydroxyl pyruvate reductase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTTACTTTGCTTGCAACACACCCAACA |
| Internal bar code: | GCTATTATGTATGAACTCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 520 |
| LEAP-Seq percent confirming: | 98.6559 |
| LEAP-Seq n confirming: | 367 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCGCACTCACAGTAGATT |
| Suggested primer 2: | CCCACTGCGTAGTTGCTGTA |