| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173379 |
| Chromosome: | chromosome 17 |
| Location: | 3542058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725350 | PSL4,GLC2B | Glucosidase IIb; (1 of 1) K08288 - protein kinase C substrate 80K-H (PRKCSH) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACATTGCTGCCGACAGTTTTTACACAT |
| Internal bar code: | GGCCCAATGCAGAATCGTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 875 |
| LEAP-Seq percent confirming: | 99.5279 |
| LEAP-Seq n confirming: | 1265 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGTGCGGTGCTATGCTC |
| Suggested primer 2: | CAAGACCATGTTGTTCACGG |