Insertion junction: LMJ.RY0402.173406_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173406
Chromosome:chromosome 9
Location:2481567
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g391150 DIV153 (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGATACCTCCCGCCATGACTCCTGGGGC
Internal bar code:TTACGCGCCTGCGCCTGCGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:350
LEAP-Seq percent confirming:99.5767
LEAP-Seq n confirming:941
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCGTGACCAGGTTGTGA
Suggested primer 2:GACCCGCACTCCAACTACAT