Insertion junction: LMJ.RY0402.173407_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.173407
Chromosome:chromosome 10
Location:1306163
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g427453 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGCCAGCTGCGGCGGCTCGCCACCAGC
Internal bar code:AACGTTCGTCTTTAGATCACT

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:1593
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGCACTACTAGCTCCGC
Suggested primer 2:CATCAACGGCAACCTGTATG