| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.173424 |
| Chromosome: | chromosome 11 |
| Location: | 1589222 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467760 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAAGCCACGTCGTTTCTCACTCCTTGCG |
| Internal bar code: | CTGACGATACGCCAGCCACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 99.686 |
| LEAP-Seq n confirming: | 2857 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGGAACATCAGTTGTGAA |
| Suggested primer 2: | TAGCGGCCTGCTTGTTTAGT |