| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173446 |
| Chromosome: | chromosome 13 |
| Location: | 5116605 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607450 | DAK1 | Dihydroxyacetone kinase; (1 of 1) 2.7.1.28 - Triokinase / Triose kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACTCCCGCATTCCCGCTCAGTGCCCCA |
| Internal bar code: | GTTAGTCACGCGCCACTCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 39 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 104 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCAGTAGCAAACACAGGT |
| Suggested primer 2: | GAGAGATGTCAGCCCCAGAG |