| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173446 |
| Chromosome: | chromosome 16 |
| Location: | 3566734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680944 | (1 of 1) K14539 - large subunit GTPase 1 (LSG1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGAAAACCTTGAACAACTGGTGCAAA |
| Internal bar code: | CGTTGTTAGTCTCAATATCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 748 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTACGTATCCCTGTGGAGCC |
| Suggested primer 2: | ACAGTCGGCAAAGGACAAGT |