Insertion junction: LMJ.RY0402.173502_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.173502
Chromosome:chromosome 6
Location:4097151
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278219 SSA6 cilia-sensing, structure and/or assembly; (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAGACGAGGGGAGGGGACGAGGGGAGGG
Internal bar code:AGTCACTGGTACTTGGCCTGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:234
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GAACAGAAAGAAGGCGATGC
Suggested primer 2:AGCCCAAACTAAAGAGGGGA