| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.173503 |
| Chromosome: | chromosome 2 |
| Location: | 2513903 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092284 | (1 of 1) 1.13.11.19 - Cysteamine dioxygenase / Persulfurase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTTCATCAGCCCTACCACGCCGACAACC |
| Internal bar code: | GGAGTGTGGCACTGCAGTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 717 |
| LEAP-Seq percent confirming: | 99.0054 |
| LEAP-Seq n confirming: | 2190 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTCAGGAGGGAGGACT |
| Suggested primer 2: | GGCATTAGGGGAATACCGTT |