| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173534 |
| Chromosome: | chromosome 7 |
| Location: | 5115012 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g347900 | (1 of 2) IPR001680//IPR001810//IPR017986 - WD40 repeat // F-box domain // WD40-repeat-containing domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGATTGCGGTGGGACCGAACATCGAGC |
| Internal bar code: | GGCTTTAAGCGGAAGTCGGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 924 |
| LEAP-Seq percent confirming: | 91.6462 |
| LEAP-Seq n confirming: | 17158 |
| LEAP-Seq n nonconfirming: | 1564 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACGAAAAACGAACAAAA |
| Suggested primer 2: | CATGGTACCTGGCAAGGAGT |