Insertion junction: LMJ.RY0402.173572_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.173572
Chromosome:chromosome 4
Location:3625172
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g228650 (1 of 18) 3.6.3.44 - Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGTTTCCCAGTCTGTCGTGCTGCCTCAT
Internal bar code:ATTATTACGATGATCGTTTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1204
LEAP-Seq percent confirming:91.4611
LEAP-Seq n confirming:964
LEAP-Seq n nonconfirming:90
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:TTACCCTATGTCCCCTTCCC
Suggested primer 2:TGGGGATGTTTTTCACCATT