Insertion junction: LMJ.RY0402.173572_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173572
Chromosome:chromosome 16
Location:684309
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g691500 (1 of 1) K18470 - Rho GTPase-activating protein 1 (ARHGAP1, CDC42GAP) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATGATTGTAAACCCCCATCATACATGCCT
Internal bar code:AAGCCATGCTACTGGGATCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:99.5968
LEAP-Seq n confirming:247
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCTGAACCAGTAGAGAGG
Suggested primer 2:TGGATCCGAGAATCTCAACC