Insertion junction: LMJ.RY0402.173580_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173580
Chromosome:chromosome 6
Location:8424946
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g307100 AKC2 (1 of 2) PTHR10566//PTHR10566:SF72 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // PROTEIN KINASE FAMILY PROTEIN; conserved protein related to ABC1/COQ8 putative ser/thr kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGACCGATGCCTAACAGCAATGAGGGT
Internal bar code:TCATTACTGCAGACTACGATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:369
LEAP-Seq percent confirming:98.4749
LEAP-Seq n confirming:1356
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTCGGTCACAGTTGAGCA
Suggested primer 2:CAGGCTCGTAGGTTACAGGC