| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173594 |
| Chromosome: | chromosome 17 |
| Location: | 294135 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698266 | (1 of 1) K01522 - bis(5'-adenosyl)-triphosphatase (FHIT) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATCCTTCAATCGTGGTGGCCGCTCATG |
| Internal bar code: | GACAGGCAATGTCCAGATCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGCACGGACACAGAAGTG |
| Suggested primer 2: | CCAACACGGAAACAGACCTT |