| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173674 |
| Chromosome: | chromosome 11 |
| Location: | 582491 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467609 | (1 of 3) K10587 - ubiquitin-protein ligase E3 A (UBE3A, E6AP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGGCGCGGTGGATCTGGATCAGTGTG |
| Internal bar code: | GGTTTCTGAATCTAAGCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 346 |
| LEAP-Seq percent confirming: | 49.5673 |
| LEAP-Seq n confirming: | 630 |
| LEAP-Seq n nonconfirming: | 641 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGTGAACATGCCGTAGT |
| Suggested primer 2: | GCCACAAAACCACACTCCTT |