Insertion junction: LMJ.RY0402.173689_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173689
Chromosome:chromosome 10
Location:941375
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g424600 (1 of 1) PF14771 - Domain of unknown function (DUF4476) (DUF4476) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCCCTCTGCAATTCCGTTTTTACTCCGT
Internal bar code:TATTGCCGACGGTTGAAAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:99.5859
LEAP-Seq n confirming:481
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCTCTCACGCAAACCTC
Suggested primer 2:ATCTCGGGTGCTAATGGTTG