Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.173695 |
Chromosome: | chromosome_17 |
Location: | 1629035 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GATTAGTGAGGCGCACAGCATCGCGTACCC |
Internal bar code: | ACCGTCGGGCCGGGTGGTGGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 998 |
LEAP-Seq percent confirming: | 80.1407 |
LEAP-Seq n confirming: | 1481 |
LEAP-Seq n nonconfirming: | 367 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAACGCTCTCAGGTGGAAC |
Suggested primer 2: | ATGGACGTCTTTGACTCGCT |