| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173696 |
| Chromosome: | chromosome 8 |
| Location: | 4155158 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379950 | PFH17,P4H17,PHX12 | Prolyl 4-hydroxylase 17; (1 of 2) PTHR10869//PTHR10869:SF79 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTAGCTTGCAGTTGCTCGCGCACAAATA |
| Internal bar code: | GTACCGAGTAGTGAGCTTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 207 |
| LEAP-Seq percent confirming: | 59.964 |
| LEAP-Seq n confirming: | 1333 |
| LEAP-Seq n nonconfirming: | 890 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCAGGTACACCAGCAG |
| Suggested primer 2: | GGCAGGTACATTGGTGCTTT |