| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.173712 |
| Chromosome: | chromosome 7 |
| Location: | 6400124 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357900 | (1 of 1) K08054 - calnexin (CANX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACTAATGCGTTCGTAGACCCGAGCGGAA |
| Internal bar code: | GTGCAGGTGCATCCGGCATGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 99.6642 |
| LEAP-Seq n confirming: | 1781 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTTTCAGTGTGTCGAGT |
| Suggested primer 2: | GGCCGCTTAGTATGAGCAAG |