Insertion junction: LMJ.RY0402.173715_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.173715
Chromosome:chromosome 8
Location:901314
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361450 (1 of 1) IPR008867//IPR009836 - Thiazole biosynthesis // Protein of unknown function DUF1399 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCGCCAGCGCCGCCAGTGCCGAGCAGCT
Internal bar code:TCGGGCACGGAGTTCCTAACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:97
LEAP-Seq percent confirming:50.5376
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:92
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GATTGGACAGCGGTATGGAC
Suggested primer 2:TGCTGCTGTTGAAGGTTTTG