Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.173718 |
Chromosome: | chromosome_9 |
Location: | 3665246 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g390578 | FAP11 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTACCGCAGACCCATTGCGTCCAGCTCCGC |
Internal bar code: | AATATAGCCTCGAAACGAATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 488 |
LEAP-Seq percent confirming: | 99.7499 |
LEAP-Seq n confirming: | 3590 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCCCTGTACAAGCGAAGAA |
Suggested primer 2: | ATAACAGACACGAGGACGGG |