| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173726 |
| Chromosome: | chromosome 6 |
| Location: | 3100386 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g274950 | (1 of 1) K18953 - factor associated with neutral sphingomyelinase activation (NSMAF, FAN) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCAACTCCTCCCCTTGATCTGCTGCAAC |
| Internal bar code: | TTTTCGGAGCGCCAGGGGAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 200 |
| LEAP-Seq percent confirming: | 99.6161 |
| LEAP-Seq n confirming: | 519 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCCTTCGACCTGTCTCG |
| Suggested primer 2: | CTAGCATGTCGCAACCTCAA |