Insertion junction: LMJ.RY0402.173735_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173735
Chromosome:chromosome 6
Location:1547794
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g260100 UAA3 UDP-galactose transporter; (1 of 2) K15276 - solute carrier family 35 (adenosine 3'-phospho 5'-phosphosulfate transporter), member B2 (SLC35B2, PAPST1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGTTGGGCAGGGGTGGTGTTGGGTGAG
Internal bar code:GACGATCACGAGTATCGTCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:266
LEAP-Seq percent confirming:56.7621
LEAP-Seq n confirming:1448
LEAP-Seq n nonconfirming:1103
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GTATTACCAGGGCTTTGCCA
Suggested primer 2:TGTCTGTACATCTCAGCGCC