Insertion junction: LMJ.RY0402.173737_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.173737
Chromosome:chromosome 7
Location:6135496
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g355750 (1 of 1) IPR001680//IPR001810//IPR017986//IPR020472 - WD40 repeat // F-box domain // WD40-repeat-containing domain // G-protein beta WD-40 repeat 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAAACGAACACATCATCATGATCTTGTG
Internal bar code:AAGATAATCATAGAACATATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:388
LEAP-Seq percent confirming:99.4273
LEAP-Seq n confirming:3646
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTCTGACAATCAAGACCA
Suggested primer 2:ATGACAGTTACAGGCCCCAG