Insertion junction: LMJ.RY0402.173738_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.173738
Chromosome:chromosome 16
Location:2078462
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g657450 MPA12 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) 3.6.1.13//3.6.1.16//3.6.1.53 - ADP-ribose diphosphatase / ADPR-PPase // CDP-glycerol diphosphatase / CDP-glycerol pyrophosphatase // Mn(2+)-dependent ADP-ribose/CDP-alcohol diphosphatase / Mn(2+)-dependent ADP-ribose/CDP-alcohol pyrophosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGTCCTGTATGACTCTCCACCTTGGACC
Internal bar code:GGGCGAGGCCGGAATCGGGTATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3
LEAP-Seq percent confirming:91.7105
LEAP-Seq n confirming:697
LEAP-Seq n nonconfirming:63
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTAAATTGACTGGCTTGGGG
Suggested primer 2:ACGGAGGGAGAGGAGTCATT