| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173827 |
| Chromosome: | chromosome 10 |
| Location: | 5789500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461350 | BUG23 | Basal-body proteins with upregulated genes 23 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCAGGGCAGAGCCCAACTTCGCACGGCGC |
| Internal bar code: | GGCGAAAGAGGGCAAAAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 944 |
| LEAP-Seq percent confirming: | 99.4048 |
| LEAP-Seq n confirming: | 167 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGTGCTGTGTACGTGGAT |
| Suggested primer 2: | ACAGCCAGAGCGAAATCTGT |