Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.173831 |
Chromosome: | chromosome 13 |
Location: | 3884592 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g590300 | URK2,CMPK1 | (1 of 1) PTHR10285:SF76 - URIDINE KINASE; Uridine kinase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAGTGCAAGGACCTGATGCAATCAGTGG |
Internal bar code: | TTCCGTCCTTCGTTCGATTCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 761 |
LEAP-Seq percent confirming: | 99.5865 |
LEAP-Seq n confirming: | 4576 |
LEAP-Seq n nonconfirming: | 19 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCACTACTCGTCCGGCTTA |
Suggested primer 2: | CTTGTCCCTTCTTGCTACGC |