Insertion junction: LMJ.RY0402.173840_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173840
Chromosome:chromosome 6
Location:3564365
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278118 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCACCGCCTGAAGCCCTGAGCTAGTAG
Internal bar code:TGGCCGGTCATTGGCTCGACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:125
LEAP-Seq percent confirming:99.7783
LEAP-Seq n confirming:1350
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTACGGTACGCCTGTGTG
Suggested primer 2:TGATTGCTTCGTGAGTTTCG