| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.173841 |
| Chromosome: | chromosome 9 |
| Location: | 4663138 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396735 | (1 of 1) PTHR18895:SF3 - METHYLTRANSFERASE-LIKE PROTEIN 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTCGTGCCTGTACGACATGCATGCATG |
| Internal bar code: | AGCGTTGACCGGCCACCAGTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 99.7394 |
| LEAP-Seq n confirming: | 4593 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTTGACCACGAAGTCCT |
| Suggested primer 2: | CGTGAAATGGCGAGTGTATG |