| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173930 |
| Chromosome: | chromosome 1 |
| Location: | 191683 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g001300 | ankryin repeat containing protein; (1 of 1) IPR000782//IPR006626 - FAS1 domain // Parallel beta-helix repeat | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGCGTACAGGAAGCCGCCGCTGCCTGC |
| Internal bar code: | GCGGCGAGTGGCATTTGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 799 |
| LEAP-Seq percent confirming: | 94.0946 |
| LEAP-Seq n confirming: | 10086 |
| LEAP-Seq n nonconfirming: | 633 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGTGTGTTTGTGTGGC |
| Suggested primer 2: | CGTGTGTGTGAGCTTGTGTG |