Insertion junction: LMJ.RY0402.173931_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173931
Chromosome:chromosome 9
Location:1976617
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394800 MITC16, MCP16 Mitochondrial substrate carrier protein; (1 of 3) K05863 - solute carrier family 25 (mitochondrial adenine nucleotide translocator), member 4/5/6/31 (SLC25A4S, ANT) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCACCGACGCCACACGCTCCCGCTCACA
Internal bar code:GGCGCAGGTGGCGAAGAGCACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1012
LEAP-Seq percent confirming:99.6458
LEAP-Seq n confirming:3376
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TGCGCACTGATTAAAAGCAC
Suggested primer 2:AAACTACCACTGGCCGTGTC