| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173944 |
| Chromosome: | chromosome 5 |
| Location: | 1759735 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242500 | PF1,RSP4 | (1 of 2) PF04712 - Radial spokehead-like protein (Radial_spoke); Radial spoke protein 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCAGGAGGTCCACCGCATTCTTTGGCT |
| Internal bar code: | TCTGAGCAGTCCGTAACGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1040 |
| LEAP-Seq percent confirming: | 99.8713 |
| LEAP-Seq n confirming: | 3105 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATCTAGCTCAGCCCCTG |
| Suggested primer 2: | GCCATTCTTCGAAGTTGCTC |