| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173948 |
| Chromosome: | chromosome 7 |
| Location: | 422250 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315200 | NRM3,NRAMP3,RET1 | Related to Ethylene Insensitive Protein 2 (EIN2); (1 of 1) PTHR11706//PTHR11706:SF45 - MANGANESE TRANSPORTER // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTGGTGGTCGCCTCCAACCTCGTCGCC |
| Internal bar code: | ATTCTAAACCGGCGGATGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 446 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTCGGTCGAGGAGAACA |
| Suggested primer 2: | CAAGCTTTGGCAAGTCAACA |