Insertion junction: LMJ.RY0402.173957_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.173957
Chromosome:chromosome_9
Location:5021549
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g398104 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):ATACATACGCCAGCACACGGTATCGCGTGA
Internal bar code:GGATATCAGCTAGCGCCGCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1016
LEAP-Seq percent confirming:89.1509
LEAP-Seq n confirming:189
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GAAATCGCTAACCAGCAAGC
Suggested primer 2:TACATGCTACCGAAGTTGCG