Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.173957 |
Chromosome: | chromosome_9 |
Location: | 5021549 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g398104 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATACATACGCCAGCACACGGTATCGCGTGA |
Internal bar code: | GGATATCAGCTAGCGCCGCGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1016 |
LEAP-Seq percent confirming: | 89.1509 |
LEAP-Seq n confirming: | 189 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAAATCGCTAACCAGCAAGC |
Suggested primer 2: | TACATGCTACCGAAGTTGCG |