| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.173960 |
| Chromosome: | scaffold 24 |
| Location: | 82345 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755697 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTTGCCCACGGGGATTGCCCCTGGCGT |
| Internal bar code: | GCTCGCTCGCTCCGCGGCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 247 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGACACCTCCGCTACC |
| Suggested primer 2: | GTGATTGCCCTTCCTGTGTT |