| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.173964 |
| Chromosome: | chromosome 3 |
| Location: | 3112797 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164900 | (1 of 1) PTHR24361//PTHR24361:SF159 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE OSR1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCCCGCTGCGGATCTGCCGCCGGCGCC |
| Internal bar code: | CTGCGTTGGGGATCTATGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 934 |
| LEAP-Seq percent confirming: | 98.6559 |
| LEAP-Seq n confirming: | 2202 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATAGAGGTGTTGGCGAGC |
| Suggested primer 2: | GCACCCAAGAAATGTCCAGT |