Insertion junction: LMJ.RY0402.173999_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.173999
Chromosome:chromosome 1
Location:4195848
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g027800 ATPVH1,ATPvH (1 of 1) K02144 - V-type H+-transporting ATPase subunit H (ATPeV1H); Vacuolar ATP synthase subunit H intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTGTAGGGGTCTGGCGACTGTGCGCCTTG
Internal bar code:CAGCGATTTAACGGCTGATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:698
LEAP-Seq percent confirming:99.7474
LEAP-Seq n confirming:2764
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCAGGAACAGGCTGATGT
Suggested primer 2:CTTTTCTCCAGCAAGCCATC