| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174012 |
| Chromosome: | chromosome 3 |
| Location: | 7282227 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207150 | (1 of 1) 1.13.12.3 - Tryptophan 2-monooxygenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCGTACTATCATAGGTGAAGTTGATCT |
| Internal bar code: | AGAAGGCATCCCGTTTCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 99.9049 |
| LEAP-Seq n confirming: | 1051 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCGTAACTCTCCTTCCGT |
| Suggested primer 2: | CAATCTTACATGCAATGCGG |