| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.174023 |
| Chromosome: | chromosome 12 |
| Location: | 8608011 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547200 | PTK6 | Putative tyrosine kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGCTCGGCGGACTGGCGGCGTCCAGC |
| Internal bar code: | GGATTTATCCCCGTGCCATGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 98.6985 |
| LEAP-Seq n confirming: | 1365 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGTTTTGCTAGCTGTGC |
| Suggested primer 2: | GTGAAGACAGGAGGAGTCCG |