| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174025 |
| Chromosome: | chromosome 9 |
| Location: | 449293 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g404503 | (1 of 3) K16489 - protein SFI1 (SFI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCAGAGGGCCTGCATTGGGCCCCGCTT |
| Internal bar code: | ACATTAGATCCGGGGCAAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1064 |
| LEAP-Seq percent confirming: | 99.5973 |
| LEAP-Seq n confirming: | 2968 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATAGTCGTCCAGCAGTCG |
| Suggested primer 2: | GATGTGTGTATGCTGACGGG |