| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174026 |
| Chromosome: | chromosome_7 |
| Location: | 4063377 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g340400 | RSP17 | Radial spoke protein 17 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTTGCCTACCTGGTTTAAGCCCCACCCCAC |
| Internal bar code: | TCTCGTGGTTTTTCGTTTCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 97.9432 |
| LEAP-Seq n confirming: | 6143 |
| LEAP-Seq n nonconfirming: | 129 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCCAATTACCGACTCAT |
| Suggested primer 2: | TAACAAGGTCCCAGGCAATC |