| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174033 |
| Chromosome: | chromosome 7 |
| Location: | 2814979 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331750 | (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTAGCTCGGGTTTGGTTGCGTTTGAGG |
| Internal bar code: | ACGGTTAGAAGGTACAGGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 129 |
| LEAP-Seq percent confirming: | 86.1227 |
| LEAP-Seq n confirming: | 1235 |
| LEAP-Seq n nonconfirming: | 199 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACTTGAGGGGAGGCAGC |
| Suggested primer 2: | GGCAGGACATGGAAACTTGT |