| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174049 |
| Chromosome: | chromosome 8 |
| Location: | 67323 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358522 | CEP5 | Cysteine endopeptidase; (1 of 1) PTHR12411//PTHR12411:SF372 - CYSTEINE PROTEASE FAMILY C1-RELATED // PROTEIN TAG-329 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTGGAGCGCGCACGCGAGCTTGTTTAC |
| Internal bar code: | GCACGTGGCAAGATGCAGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 97.4328 |
| LEAP-Seq n confirming: | 1594 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCGTAACACCAATCGAAC |
| Suggested primer 2: | GTTGTCCGACCACCTCTGAT |