| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174074 |
| Chromosome: | chromosome 9 |
| Location: | 1985409 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394700 | RNZ1 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z; Ribonuclease Z | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAACCCCAGCACCCACCCACCCTCTCCA |
| Internal bar code: | GTCCGATCGCGGTGTGCTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 81.1908 |
| LEAP-Seq n confirming: | 600 |
| LEAP-Seq n nonconfirming: | 139 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTCCGCATTTTCCTCACC |
| Suggested primer 2: | CATATGGGCGTATGTAGGGG |