| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.174074 |
| Chromosome: | chromosome 9 |
| Location: | 1985444 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394700 | RNZ1 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z; Ribonuclease Z | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTGGCATGGGTTAGGCGCGGCTAGCAGC |
| Internal bar code: | GGGGTCTGATCAACAAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 80.563 |
| LEAP-Seq n confirming: | 1803 |
| LEAP-Seq n nonconfirming: | 435 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATGGGCGTATGTAGGGG |
| Suggested primer 2: | TATTCCGCATTTTCCTCACC |