| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174096 |
| Chromosome: | chromosome 17 |
| Location: | 3567486 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725550 | GLD1 | Glucose-6-phosphate-1-dehydrogenase; (1 of 2) 1.1.1.49 - Glucose-6-phosphate dehydrogenase (NADP(+)) / GPD | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTATGAAGGGGGAGGGGGGCGGGGTTGTA |
| Internal bar code: | AGACGGGTTGGCGCTACCGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 594 |
| LEAP-Seq percent confirming: | 61.8469 |
| LEAP-Seq n confirming: | 18739 |
| LEAP-Seq n nonconfirming: | 11560 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTCATCCAGAACCACCT |
| Suggested primer 2: | AAAGGGAGGGAGCGTTGTAT |