| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.174102 |
| Chromosome: | chromosome_10 |
| Location: | 5095524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g456100 | AGG3 | Flagellar flavodoxin, involved in phototaxis | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGCTGACGCACATGCTGTTGCCGCCTCTC |
| Internal bar code: | GGCCCAGTCGGACTTAAGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1121 |
| LEAP-Seq percent confirming: | 99.3737 |
| LEAP-Seq n confirming: | 2380 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCCGTCCACTCTGTCATT |
| Suggested primer 2: | ACCCGTGTGATGCTGTCATA |