| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174113 |
| Chromosome: | chromosome 13 |
| Location: | 4183149 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592200 | GLT1,GSN1 | Glutamate synthase, NADH-dependent; (1 of 1) 1.4.1.14 - Glutamate synthase (NADH) / NADH-glutamate synthase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTGATGAACGCCCACGTGGGCGGCAAC |
| Internal bar code: | GCCAGTTGCCTTGGGATCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.8681 |
| LEAP-Seq n confirming: | 757 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGAACTACGACCGCAAG |
| Suggested primer 2: | GCAGGTTTGGCATTACAGGT |