| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.174133 |
| Chromosome: | chromosome 7 |
| Location: | 2243040 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327350 | CDPK8 | Calcium/calmodulin-dependent protein kinase; (1 of 1) K04515 - calcium/calmodulin-dependent protein kinase (CaM kinase) II (CAMK2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCCAGTCGCCGCTTCCAAGCGCCCTGCC |
| Internal bar code: | AAATGTGGGCCGGGGGAGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.7534 |
| LEAP-Seq n confirming: | 2832 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCGTGGTGTATATCGTG |
| Suggested primer 2: | ATGTTTCCGCTATTGCCATC |