Insertion junction: LMJ.RY0402.174137_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.174137
Chromosome:chromosome 4
Location:2357231
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g220850 (1 of 1) 3.6.3.34//3.6.3.43//3.6.3.44 - Iron-chelate-transporting ATPase // Peptide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTGATGGGCAGACAGAAATCGCTTGGT
Internal bar code:GGGCCGCCTGGAAACGACCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:960
LEAP-Seq percent confirming:98.5465
LEAP-Seq n confirming:678
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGGTGCTGGTTTTGTT
Suggested primer 2:CGAGTCAATCCAGCAGTCAA